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Home>Product>Primary Antibody

KD-Validated ATP5F1B Recombinant Rabbit mAb

#66878

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using ATP5F1B antibody (Cat#66878). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP5F1B antibody (Cat#66878, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ATP5F1B antibody (Cat#66878). ATP5F1B expression in wild type (WT) and ATP5F1B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATP5F1B antibody (Cat#66878, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol：
    ATP5F1B
  • Catalog：
    66878
  • Application：
    WB
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated ATP5F1B Recombinant Rabbit mAb
Aliases ATP5F1B; ATP Synthase F1 Subunit Beta; ATP5B; ATPSB; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Beta Polypeptide; ATP Synthase F(1) Complex Subunit Beta, Mitochondrial; ATPMB; Mitochondrial ATP Synthetase, Beta Subunit; ATP Synthase Subunit Beta, Mitochondrial; Mitochondrial ATP Synthase Beta Subunit; Epididymis Secretory Protein Li 271; EC 3.6.3.14; EC 7.1.2.2; HEL-S-271; EC 3.6.3; HUMOP2
Background

Gene Name: ATP5F1B

NCBI Gene Entry: 506

UniProt Entry: P06576

Application Information

Molecular Weight: Predicted, 57 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB1140

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human ATPB
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Note This product is for research use only.
Data
  • Western blotting analysis using ATP5F1B antibody (Cat#66878). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP5F1B antibody (Cat#66878, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using ATP5F1B antibody (Cat#66878). ATP5F1B expression in wild type (WT) and ATP5F1B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATP5F1B antibody (Cat#66878, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP5F1B
  • Catalog：
    66878
  • Application：
    WB
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ATP5F1B antibody (Cat#66878). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP5F1B antibody (Cat#66878, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ATP5F1B antibody (Cat#66878). ATP5F1B expression in wild type (WT) and ATP5F1B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATP5F1B antibody (Cat#66878, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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