KD-Validated MARK3 Recombinant Rabbit mAb - Product

KD-Validated MARK3 Recombinant Rabbit mAb

#61649

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Recombinant Antibody
Knockdown Validated

Western blotting analysis using MARK3 antibody (Cat#61649). MARK3 expression in wild type (WT) and MARK3 knockdown (KD) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with MARK3 antibody (Cat#61649, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of MARK3 expression in HT-1080 cells using MARK3 antibody (Cat#61649, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MARK3.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

MARK3
Catalog：

61649
Application：

WB, FCM
Species Reactivity：

H

Information

Data

Information

Product Name	KD-Validated MARK3 Recombinant Rabbit mAb
Aliases	MARK3; Microtubule Affinity Regulating Kinase 3; CTAK1; MAP/Microtubule Affinity-Regulating Kinase 3; PAR-1A; KP78; Serine/Threonine-Protein Kinase P78; Cdc25C-Associated Protein Kinase 1; Ser/Thr Protein Kinase PAR-1; Protein Kinase STK10; ELKL Motif Kinase 2; EC 2.7.11.1; C-TAK1; EMK-2; EC 2.7.11; Par-1a; PAR1A; VIPB; EMK2
Background	Gene Name: MARK3 NCBI Gene Entry: 4140 UniProt Entry: P27448
Application Information	Molecular Weight: Predicted, 84 kDa; observed, 75 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB3140 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human MARK3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using MARK3 antibody (Cat#61649). MARK3 expression in wild type (WT) and MARK3 knockdown (KD) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with MARK3 antibody (Cat#61649, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of MARK3 expression in HT-1080 cells using MARK3 antibody (Cat#61649, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MARK3.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

MARK3
Catalog：

61649
Application：

WB, FCM
Species Reactivity：

H

Datasheet

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