KD-Validated BNIP3 Recombinant Rabbit mAb - Product

KD-Validated BNIP3 Recombinant Rabbit mAb

#64885

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Recombinant Antibody
Knockdown Validated

Western blotting analysis using BNIP3 antibody (Cat#64885). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BNIP3 antibody (Cat#64885, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using BNIP3 antibody (Cat# 64885). BNIP3 expression in wild-type (WT) and BNIP3 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BNIP3 antibody (Cat# 64885, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat# 201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat# 716).

Flow cytometric analysis of BNIP3 expression in HepG2 cells using BNIP3 antibody (Cat#64885, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BNIP3.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

BNIP3
Catalog：

64885
Application：

WB, FCM
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KD-Validated BNIP3 Recombinant Rabbit mAb
Aliases	BNIP3; BCL2 Interacting Protein 3; HABON; Nip3; BCL2/Adenovirus E1B 19 KDa Protein-Interacting Protein 3; Hypoxia-Activated BNIP3 Overlapping Non-Coding RNA; BCL2/Adenovirus E1B 19kDa Interacting Protein 3; Nineteen KD Interacting Protein-3; BCL2/Adenovirus E1B Interacting Protein 3; NIP3
Background	Gene Name: BNIP3 NCBI Gene Entry: 664 UniProt Entry: Q12983
Application Information	Molecular Weight: Predicted, 22 kDa; observed, 24-30 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB13585 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human BNIP3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:100-1:1,000
Note	This product is for research use only.

Data

Western blotting analysis using BNIP3 antibody (Cat#64885). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BNIP3 antibody (Cat#64885, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using BNIP3 antibody (Cat# 64885). BNIP3 expression in wild-type (WT) and BNIP3 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BNIP3 antibody (Cat# 64885, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat# 201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat# 716).
Flow cytometric analysis of BNIP3 expression in HepG2 cells using BNIP3 antibody (Cat#64885, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BNIP3.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

BNIP3
Catalog：

64885
Application：

WB, FCM
Species Reactivity：

H, M, R

Datasheet

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