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Home>Product>Primary Antibody

KD-Validated OLIG1 Recombinant Rabbit mAb

#61745

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using OLIG1 antibody (Cat#61745). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with OLIG1antibody (Cat#61745, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using OLIG1 antibody (Cat#61745). OLIG1 expression in wild-type (WT) and OLIG1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with OLIG1 antibody (Cat#61745, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of OLIG1 expression in HepG2 cells using OLIG1 antibody (Cat#61745, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, OLIG1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    OLIG1
  • Catalog：
    61745
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated OLIG1 Recombinant Rabbit mAb
Aliases OLIG1; Oligodendrocyte Transcription Factor 1; BHLHB6; BHLHE21; Oligodendrocyte-Specific BHLH Transcription Factor 1; Basic Domain, Helix-Loop-Helix Protein, Class B, 6; Oligodendrocyte Lineage Transcription Factor 1; Class E Basic Helix-Loop-Helix Protein 21; Class B Basic Helix-Loop-Helix Protein 6; BHLHe21; Oligo1; BHLHb6
Background

Gene Name: OLIG1

NCBI Gene Entry: 116448

UniProt Entry: Q8TAK6

Application Information

Molecular Weight: Predicted, 28 kDa; observed, 35 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB3520

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human Olig1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using OLIG1 antibody (Cat#61745). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with OLIG1antibody (Cat#61745, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using OLIG1 antibody (Cat#61745). OLIG1 expression in wild-type (WT) and OLIG1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with OLIG1 antibody (Cat#61745, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of OLIG1 expression in HepG2 cells using OLIG1 antibody (Cat#61745, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, OLIG1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    OLIG1
  • Catalog：
    61745
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using OLIG1 antibody (Cat#61745). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with OLIG1antibody (Cat#61745, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using OLIG1 antibody (Cat#61745). OLIG1 expression in wild-type (WT) and OLIG1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with OLIG1 antibody (Cat#61745, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of OLIG1 expression in HepG2 cells using OLIG1 antibody (Cat#61745, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, OLIG1.
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