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Home>Product>Primary Antibody

KD-Validated CDH2 Mouse mAb

#61779

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Knockdown Validated
Western blotting analysis using CDH2 antibody (Cat#61779). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDH2 antibody (Cat#61779, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CDH2 antibody (Cat#61779). CDH2 expression in wild-type (WT) and CDH2 knockdown (KD) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with CDH2 antibody (Cat#61779, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using CDH2 antibody (Cat#61779, 1:150). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CDH2
  • Catalog：
    61779
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated CDH2 Mouse mAb
Aliases CDH2; Cadherin 2; CDHN; CD325; NCAD; Cadherin 2, Type 1, N-Cadherin (Neuronal); Neural Cadherin; N-Cadherin; Cadherin-2; CDw325; Calcium-Dependent Adhesion Protein, Neuronal; CD325 Antigen; N-Cadherin 1; ARVD14; ACOGS; ADHD8
Background

Gene Name: CDH2

NCBI Gene Entry: 1000

UniProt Entry: P19022

Application Information

Molecular Weight: Predicted, 100 kDa; observed, 68-80 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB3720

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunohistochemistry-Paraffin (IHC-P)
Immunogen Recombinant protein of human CDH2
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:2,500
Immunohistochemistry-Paraffin (IHC-P): 1:75-1:150
Note This product is for research use only.
Data
  • Western blotting analysis using CDH2 antibody (Cat#61779). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDH2 antibody (Cat#61779, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using CDH2 antibody (Cat#61779). CDH2 expression in wild-type (WT) and CDH2 knockdown (KD) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with CDH2 antibody (Cat#61779, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using CDH2 antibody (Cat#61779, 1:150). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CDH2
  • Catalog：
    61779
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using CDH2 antibody (Cat#61779). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDH2 antibody (Cat#61779, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CDH2 antibody (Cat#61779). CDH2 expression in wild-type (WT) and CDH2 knockdown (KD) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with CDH2 antibody (Cat#61779, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using CDH2 antibody (Cat#61779, 1:150). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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