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Home>Product>Primary Antibody

KD-Validated Phospho-Rad17 (S656) Recombinant Rabbit mAb

#62429

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using Phospho-Rad17 (S656) antibody (Cat#62429). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Rad17 (S656) antibody (Cat#62429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using phospho-Rad17 (S656) antibody (Cat#62429). Phospho-Rad17 (S656) expression in wild-type (WT) and RAD17 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with phospho-Rad17 (S656) antibody (Cat#62429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with Phospho-Rad17 (S656) antibody (Cat#62429, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Rad17 (S656) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using Phospho-Rad17 (S656) antibody (Cat#62429, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-Rad17 (S656) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-Rad17 (S656) was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Phospho-Rad17 (S656) expression in HAP-1 cells using Phospho-Rad17 (S656) antibody (Cat#62429, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Rad17 (S656).
Validation of Rad17 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-Rad17 (S656) antibody (Cat#62429, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    RAD17
  • Catalog：
    62429
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name KD-Validated Phospho-Rad17 (S656) Recombinant Rabbit mAb
Aliases RAD17 Checkpoint Clamp Loader Component; RAD17Sp; Rad24; CCYC; Cell Cycle Checkpoint Protein RAD17; R24L; Cell Cycle Checkpoint Protein (RAD17); RAD17 Homolog (S. Pombe); RF-C Activator 1 Homolog; RF-C/Activator 1 Homolog; RAD1 (S. Pombe) Homolog; Rad17-Like Protein; RAD17 Homolog; RAD1 Homolog; HRAD17; HRad17
Background

Gene Name: RAD17

NCBI Gene Entry: 5884

UniProt Entry: O75943

Application Information

Molecular Weight: Predicted, 77 kDa; observed, 85 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB795

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-Rad17 (S656)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Phospho-Rad17 (S656) antibody (Cat#62429). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Rad17 (S656) antibody (Cat#62429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using phospho-Rad17 (S656) antibody (Cat#62429). Phospho-Rad17 (S656) expression in wild-type (WT) and RAD17 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with phospho-Rad17 (S656) antibody (Cat#62429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with Phospho-Rad17 (S656) antibody (Cat#62429, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Rad17 (S656) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Immunocytochemical staining of HeLa cells using Phospho-Rad17 (S656) antibody (Cat#62429, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-Rad17 (S656) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-Rad17 (S656) was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of Phospho-Rad17 (S656) expression in HAP-1 cells using Phospho-Rad17 (S656) antibody (Cat#62429, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Rad17 (S656).
  • Validation of Rad17 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-Rad17 (S656) antibody (Cat#62429, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RAD17
  • Catalog：
    62429
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using Phospho-Rad17 (S656) antibody (Cat#62429). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Rad17 (S656) antibody (Cat#62429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using phospho-Rad17 (S656) antibody (Cat#62429). Phospho-Rad17 (S656) expression in wild-type (WT) and RAD17 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with phospho-Rad17 (S656) antibody (Cat#62429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with Phospho-Rad17 (S656) antibody (Cat#62429, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Rad17 (S656) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using Phospho-Rad17 (S656) antibody (Cat#62429, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-Rad17 (S656) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-Rad17 (S656) was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Phospho-Rad17 (S656) expression in HAP-1 cells using Phospho-Rad17 (S656) antibody (Cat#62429, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Rad17 (S656).
Validation of Rad17 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-Rad17 (S656) antibody (Cat#62429, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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