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Home>Product>Primary Antibody

KD-Validated GOLGA1 Recombinant Rabbit mAb

#61982

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using GOLGA1 antibody (Cat.#61982). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GOLGA1 antibody (Cat.#61982, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using GOLGA1 antibody (Cat#61982). GOLGA1 expression in wild-type (WT) and GOLGA1 shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with GOLGA1 antibody (Cat#61982, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon GOLGA1 antibody (Cat#61982, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    GOLGA1
  • Catalog：
    61982
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated GOLGA1 Recombinant Rabbit mAb
Aliases GOLGA1; Golgin A1; Golgin-97; Golgi Autoantigen, Golgin Subfamily A, 1; Golgin Subfamily A Member 1; MGC33154; Gap Junction Protein, Alpha 4, 37kD; GOLGIN-97
Background

Gene Name: GOLGA1

NCBI Gene Entry: 2800

UniProt Entry: Q92805

Application Information

Molecular Weight: Predicted, 88 kDa; observed, 97 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB6810

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human GOLGA1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using GOLGA1 antibody (Cat.#61982). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GOLGA1 antibody (Cat.#61982, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using GOLGA1 antibody (Cat#61982). GOLGA1 expression in wild-type (WT) and GOLGA1 shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with GOLGA1 antibody (Cat#61982, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human sigmoid colon GOLGA1 antibody (Cat#61982, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    GOLGA1
  • Catalog：
    61982
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using GOLGA1 antibody (Cat.#61982). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GOLGA1 antibody (Cat.#61982, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using GOLGA1 antibody (Cat#61982). GOLGA1 expression in wild-type (WT) and GOLGA1 shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with GOLGA1 antibody (Cat#61982, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon GOLGA1 antibody (Cat#61982, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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