Western blotting analysis using mannose phosphate isomerase antibody (Cat#61789). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with mannose phosphate isomerase antibody (Cat#61789, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using mannose phosphate isomerase antibody (Cat#61789). Mannose phosphate isomerase expression in wild-type (WT) and mannose phosphate isomerase (MPI) knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with mannose phosphate isomerase antibody (Cat#61789, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Mannose phosphate isomerase expression in HeLa cells using Mannose phosphate isomerase antibody (Cat#61789, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Mannose phosphate isomerase.
