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Home>Product>Primary Antibody

KD-Validated MAPK9 Mouse mAb

#61790

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Knockdown Validated
Western blotting analysis using mitogen-activated protein kinase 9 antibody (Cat#61790). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with mitogen-activated protein kinase 9 antibody (Cat#61790, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using mitogen-activated protein kinase 9 antibody (Cat#61790). Mitogen-activated protein kinase 9 expression in wild-type (WT) and mitogen-activated protein kinase 9 (MAPK9) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with mitogen-activated protein kinase 9 antibody (Cat#61790, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Mitogen-activated protein kinase 9 expression in HeLa cells using Mitogen-activated protein kinase 9 antibody (Cat#61790, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Mitogen-activated protein kinase 9.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAPK9
  • Catalog：
    61790
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated MAPK9 Mouse mAb
Aliases MAPK9; Mitogen-Activated Protein Kinase 9; JNK2; PRKM9; P54a; SAPK; Stress-Activated Protein Kinase JNK2; Stress-Activated Protein Kinase 1a; C-Jun N-Terminal Kinase; MAP Kinase 9; EC 2.7.11.24; Jun Kinase; JNK-55; SAPK1a; MAPK 9; C-Jun Kinase; JNK2ALPHA; EC 2.7.11; JNK2BETA; P54aSAPK; SAPK1A; JNK2A; JNK2B
Background

Gene Name: MAPK9

NCBI Gene Entry: 5601

UniProt Entry: P45984

Application Information

Molecular Weight: Predicted, 48 kDa; observed, 48 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB3780

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human MAPK9
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using mitogen-activated protein kinase 9 antibody (Cat#61790). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with mitogen-activated protein kinase 9 antibody (Cat#61790, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using mitogen-activated protein kinase 9 antibody (Cat#61790). Mitogen-activated protein kinase 9 expression in wild-type (WT) and mitogen-activated protein kinase 9 (MAPK9) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with mitogen-activated protein kinase 9 antibody (Cat#61790, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Mitogen-activated protein kinase 9 expression in HeLa cells using Mitogen-activated protein kinase 9 antibody (Cat#61790, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Mitogen-activated protein kinase 9.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAPK9
  • Catalog：
    61790
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using mitogen-activated protein kinase 9 antibody (Cat#61790). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with mitogen-activated protein kinase 9 antibody (Cat#61790, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using mitogen-activated protein kinase 9 antibody (Cat#61790). Mitogen-activated protein kinase 9 expression in wild-type (WT) and mitogen-activated protein kinase 9 (MAPK9) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with mitogen-activated protein kinase 9 antibody (Cat#61790, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Mitogen-activated protein kinase 9 expression in HeLa cells using Mitogen-activated protein kinase 9 antibody (Cat#61790, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Mitogen-activated protein kinase 9.
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