Western blotting analysis using developmental pluripotency associated 4 antibody (Cat#61866). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with developmental pluripotency associated 4 antibody (Cat#61866, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using developmental pluripotency associated 4 antibody (Cat#61866). Developmental pluripotency associated 4 expression in wild-type (WT) and developmental pluripotency associated 4 (DPPA4) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with developmental pluripotency associated 4 antibody (Cat#61866, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Developmental pluripotency associated 4 antibody (Cat#61866, 1:1,000). Nuclei were stained blue with DAPI; Developmental pluripotency associated 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar, 20 μm.
Flow cytometric analysis of Developmental pluripotency associated 4 expression in HAP-1 cells using Developmental pluripotency associated 4 antibody (Cat#61866, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Developmental pluripotency associated 4.
