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Home>Product>Primary Antibody

KD-Validated EEF2K Rabbit pAb

#62828

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shRNA Knockdown Validated
Western blotting analysis using EEF2K antibody (Cat#62828). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EEF2K antibody (Cat#62828, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using EEF2K antibody (Cat#62828). EEF2K expression in wild type (WT) and EEF2K shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with EEF2K antibody (Cat#62828, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    EEF2K
  • Catalog：
    62828
  • Application：
    WB
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name KD-Validated EEF2K Rabbit pAb
Aliases EEF2K; Eukaryotic Elongation Factor 2 Kinase; EEF-2K; CaMKIII; EEF-2 Kinase; EC 2.7.11.20; Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor-2 Kinase; Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase; Calmodulin-Dependent Protein Kinase III; Eukaroytic Elongation Factor 2 Kinase; Elongation Factor-2 Kinase; Alternative Protein EEF2K; EC 2.7.11; HSU93850
Background

Gene Name: EEF2K

NCBI Gene Entry: 29904

UniProt Entry: O00418

Application Information

Molecular Weight: Predicted, 82 kDa; observed, 105 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human EEF2K
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using EEF2K antibody (Cat#62828). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EEF2K antibody (Cat#62828, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using EEF2K antibody (Cat#62828). EEF2K expression in wild type (WT) and EEF2K shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with EEF2K antibody (Cat#62828, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    EEF2K
  • Catalog：
    62828
  • Application：
    WB
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using EEF2K antibody (Cat#62828). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EEF2K antibody (Cat#62828, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using EEF2K antibody (Cat#62828). EEF2K expression in wild type (WT) and EEF2K shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with EEF2K antibody (Cat#62828, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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