Western blotting analysis using annexin V antibody (Cat#4304). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with annexin V antibody (Cat#4304, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of annexin V expression in HT-1080 cells using annexin V antibody (Cat#4304, 1:2,000). Green, isotype control; red, annexin V.
Immunocytochemical staining of HT-1080 cells with ANXA5 antibody(Cat#4304, 1:1,000). Nuclei were stained blue with DAPI; ANXA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using annexin V antibody (Cat#4304). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with annexin V antibody (Cat#4304, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of annexin V expression in HT-1080 cells using annexin V antibody (Cat#4304, 1:2,000). Green, isotype control; red, annexin V.
Immunocytochemical staining of HT-1080 cells with ANXA5 antibody(Cat#4304, 1:1,000). Nuclei were stained blue with DAPI; ANXA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using annexin V antibody (Cat#4304). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with annexin V antibody (Cat#4304, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of annexin V expression in HT-1080 cells using annexin V antibody (Cat#4304, 1:2,000). Green, isotype control; red, annexin V.
Immunocytochemical staining of HT-1080 cells with ANXA5 antibody(Cat#4304, 1:1,000). Nuclei were stained blue with DAPI; ANXA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000795s ]
SELECT * FROM `goods` WHERE `code` = '4304' LIMIT 1 [ RunTime:0.000473s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000414s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000345s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000364s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5632 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000346s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000537s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5632 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000330s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000386s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000261s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.054516s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (10273,8045,7995,5984,7844,10351,5964,5490,8998,4658) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000908s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.001054s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000475s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000518s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000226s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000367s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000375s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000264s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000238s ]
