Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

KD-Validated ENOPH1 Mouse mAb

#61869

Click to copy product information

Return to List

shRNA Knockdown Validated
Western blotting analysis using ENOPH1 antibody (Cat#61869). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ENOPH1 antibody (Cat#61869, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ENOPH1 antibody (Cat#61869). ENOPH1 expression in wild type (WT) and ENOPH1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ENOPH1 antibody (Cat#61869, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ENOPH1 expression in HAP-1 cells using ENOPH1 antibody (Cat#61869, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ENOPH1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ENOPH1
  • Catalog：
    61869
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name KD-Validated ENOPH1 Mouse mAb
Aliases ENOPH1; Enolase-Phosphatase 1; MASA; Enolase-Phosphatase E1; MtnC; E1; 2,3-Diketo-5-Methylthio-1-Phosphopentane Phosphatase; Acireductone Synthase; EC 3.1.3.77; MASA Homolog; MST145
Background

Gene Name: ENOPH1

NCBI Gene Entry: 58478

UniProt Entry: Q9UHY7

Application Information

Molecular Weight: Predicted, 29 kDa; observed, 29 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB4025

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human ENOPH1
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ENOPH1 antibody (Cat#61869). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ENOPH1 antibody (Cat#61869, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using ENOPH1 antibody (Cat#61869). ENOPH1 expression in wild type (WT) and ENOPH1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ENOPH1 antibody (Cat#61869, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of ENOPH1 expression in HAP-1 cells using ENOPH1 antibody (Cat#61869, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ENOPH1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ENOPH1
  • Catalog：
    61869
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using ENOPH1 antibody (Cat#61869). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ENOPH1 antibody (Cat#61869, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ENOPH1 antibody (Cat#61869). ENOPH1 expression in wild type (WT) and ENOPH1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ENOPH1 antibody (Cat#61869, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ENOPH1 expression in HAP-1 cells using ENOPH1 antibody (Cat#61869, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ENOPH1.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.