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KD-Validated Phospho-LIPE (S855) Recombinant Rabbit mAb

#61968

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Recombinant Antibody
Knockdown Validated
Western blotting analysis using Phospho-LIPE (Ser855) antibody (Cat#61968). Phospho-LIPE (Ser855) expression in wild-type (WT) and LIPE knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Phospho-LIPE (Ser855) antibody (Cat#61968, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    LIPE
  • Catalog:
    61968
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated Phospho-LIPE (S855) Recombinant Rabbit mAb
Aliases LIPE; Lipase E, Hormone Sensitive Type; HSL; Monoacylglycerol Lipase LIPE; Lipase, Hormone-Sensitive; Hormone-Sensitive Lipase; Retinyl Ester Hydrolase; EC 3.1.1.79; REH; EC 3.1.1.23; AOMS4; FPLD6; LHS
Background

Gene Name: LIPE

NCBI Gene Entry: 3991

UniProt Entry: Q05469

Application Information

Molecular Weight: Predicted, 117 kDa; observed, 83 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB6075

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human Phospho-LIPE (Ser855)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using Phospho-LIPE (Ser855) antibody (Cat#61968). Phospho-LIPE (Ser855) expression in wild-type (WT) and LIPE knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Phospho-LIPE (Ser855) antibody (Cat#61968, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Phospho-LIPE (Ser855) antibody (Cat#61968). Phospho-LIPE (Ser855) expression in wild-type (WT) and LIPE knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Phospho-LIPE (Ser855) antibody (Cat#61968, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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