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Home>Product>Primary Antibody

KD-Validated CSNK1E mouse mAb

#61250

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shRNA Knockdown Validated
Western blotting analysis using casein kinase 1 epsilon antibody (Cat#61250). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with casein kinase 1 epsilon antibody (Cat#61250, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using casein kinase 1 epsilon antibody (Cat#61250). Casein kinase 1 epsilon expression in wild type (WT) and casein kinase 1 epsilon (CSNK1E) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with casein kinase 1 epsilon antibody (Cat#61250, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using casein kinase 1 epsilon antibody (Cat#61250, 1:1,000), Top panel: wild-type (WT); Bottom panal: Casein kinase 1 epsilon shRNA knockdown (KD). Nuclei were stained blue with DAPI; Casein kinase 1 epsilon was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HepG2 cells with casein kinase 1 epsilon antibody (Cat#61250, 1:1,000). Nuclei were stained blue with DAPI; Casein kinase 1 epsilon was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of casein kinase 1 epsilon expression in HT-1080 cells using casein kinase 1 epsilon antibody (Cat# 61250, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Casein kinase 1 epsilon.
Validation of casein kinase 1 epsilon knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with casein kinase 1 epsilon antibody (Cat#61250, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CSNK1E
  • Catalog：
    61250
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated CSNK1E mouse mAb
Aliases CSNK1E; Casein Kinase 1 Epsilon; CKIepsilon; HCKIE; CKIE; Casein Kinase I Isoform Epsilon; EC 2.7.11.1; Casein Kinase 1, Epsilon; CKI-Epsilon; EC 2.7.11; CKIe
Background

Gene Name: CSNK1E

NCBI Gene Entry: 1454

UniProt Entry: P49674

Application Information

Molecular Weight: Predicted, 47 kDa; observed, 43 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB490

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human CSNK1E
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using casein kinase 1 epsilon antibody (Cat#61250). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with casein kinase 1 epsilon antibody (Cat#61250, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using casein kinase 1 epsilon antibody (Cat#61250). Casein kinase 1 epsilon expression in wild type (WT) and casein kinase 1 epsilon (CSNK1E) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with casein kinase 1 epsilon antibody (Cat#61250, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HeLa cells using casein kinase 1 epsilon antibody (Cat#61250, 1:1,000), Top panel: wild-type (WT); Bottom panal: Casein kinase 1 epsilon shRNA knockdown (KD). Nuclei were stained blue with DAPI; Casein kinase 1 epsilon was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Immunocytochemical staining of HepG2 cells with casein kinase 1 epsilon antibody (Cat#61250, 1:1,000). Nuclei were stained blue with DAPI; Casein kinase 1 epsilon was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of casein kinase 1 epsilon expression in HT-1080 cells using casein kinase 1 epsilon antibody (Cat# 61250, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Casein kinase 1 epsilon.
  • Validation of casein kinase 1 epsilon knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with casein kinase 1 epsilon antibody (Cat#61250, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CSNK1E
  • Catalog：
    61250
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using casein kinase 1 epsilon antibody (Cat#61250). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with casein kinase 1 epsilon antibody (Cat#61250, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using casein kinase 1 epsilon antibody (Cat#61250). Casein kinase 1 epsilon expression in wild type (WT) and casein kinase 1 epsilon (CSNK1E) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with casein kinase 1 epsilon antibody (Cat#61250, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using casein kinase 1 epsilon antibody (Cat#61250, 1:1,000), Top panel: wild-type (WT); Bottom panal: Casein kinase 1 epsilon shRNA knockdown (KD). Nuclei were stained blue with DAPI; Casein kinase 1 epsilon was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HepG2 cells with casein kinase 1 epsilon antibody (Cat#61250, 1:1,000). Nuclei were stained blue with DAPI; Casein kinase 1 epsilon was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of casein kinase 1 epsilon expression in HT-1080 cells using casein kinase 1 epsilon antibody (Cat# 61250, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Casein kinase 1 epsilon.
Validation of casein kinase 1 epsilon knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with casein kinase 1 epsilon antibody (Cat#61250, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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