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Interleukin 12B Mouse mAb

#3483

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Western blotting analysis using interleukin 12B antibody (Cat#3483). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with interleukin 12B antibody (Cat#3483, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of Interleukin 12B expression in HAP-1 cells using Interleukin 12B antibody (Cat#3483, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Interleukin 12B.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    IL12B
  • Catalog:
    3483
  • Application:
    WB, FCM
  • Species Reactivity:
    H, R
Information
Product Name Interleukin 12B Mouse mAb
Aliases IL12B; Interleukin 12B; IL-12B; CLMF2; NKSF2; CLMF; NKSF; Interleukin 12B (Natural Killer Cell Stimulatory Factor 2, Cytotoxic Lymphocyte Maturation Factor 2, P40); Natural Killer Cell Stimulatory Factor, 40 KD Subunit; Cytotoxic Lymphocyte Maturation Factor 40 KDa Subunit; NK Cell Stimulatory Factor Chain; Interleukin-12 Subunit Beta; Interleukin-12 Beta Chain; Interleukin 12, P40; IL12, Subunit P40; IL-12 Subunit P40; CLMF P40; Cytotoxic Lymphocyte Maturation Factor 2, P40; Natural Killer Cell Stimulatory Factor-2; IMD28; IMD29
Background

Gene Name: IL12B

NCBI Gene Entry: 3593

UniProt Entry: P29460

Application Information

Molecular Weight: Predicted, 37 kDa; observed, 40 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB5840

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human IL12B
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB):1:2,500-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using interleukin 12B antibody (Cat#3483). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with interleukin 12B antibody (Cat#3483, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
  • Flow cytometric analysis of Interleukin 12B expression in HAP-1 cells using Interleukin 12B antibody (Cat#3483, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Interleukin 12B.
Western blotting analysis using interleukin 12B antibody (Cat#3483). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with interleukin 12B antibody (Cat#3483, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of Interleukin 12B expression in HAP-1 cells using Interleukin 12B antibody (Cat#3483, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Interleukin 12B.
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