Western blotting analysis using DNAJC7 antibody (Cat#6294). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNAJC7 antibody (Cat#6294, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DNAJC7 expression in HepG2 cells using DNAJC7 antibody (Cat#6294,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DNAJC7.
DNAJC7; DnaJ Heat Shock Protein Family (Hsp40) Member C7; TPR2; TTC2; DnaJ (Hsp40) Homolog, Subfamily C, Member 7; Tetratricopeptide Repeat Protein 2; DnaJ Homolog Subfamily C Member 7; TPR Repeat Protein 2; Tetratricopeptide Repeat Domain 2; DJ11; DJC7
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human DNAJC7
Isotype
Mouse IgG2b
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using DNAJC7 antibody (Cat#6294). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNAJC7 antibody (Cat#6294, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DNAJC7 expression in HepG2 cells using DNAJC7 antibody (Cat#6294,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DNAJC7.
Western blotting analysis using DNAJC7 antibody (Cat#6294). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNAJC7 antibody (Cat#6294, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DNAJC7 expression in HepG2 cells using DNAJC7 antibody (Cat#6294,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DNAJC7.
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