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Home>Product>Primary Antibody

Neutrophil Cytosolic Factor 4 Recombinant Rabbit mAb

#2266

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Recombinant Antibody
Western blotting analysis using Neutrophil cytosolic factor 4 antibody (Cat#2266). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Neutrophil cytosolic factor 4 antibody (Cat#2266, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with Neutrophil cytosolic factor 4 antibody(Cat#2266, 1:1,000). Nuclei were stained blue with DAPI; Neutrophil cytosolic factor 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NCF4
  • Catalog：
    2266
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Neutrophil Cytosolic Factor 4 Recombinant Rabbit mAb
Aliases Neutrophil Cytosolic Factor 4; P40phox; SH3PXD4; Neutrophil NADPH Oxidase Factor 4; SH3 And PX Domain-Containing Protein 4; Neutrophil Cytosolic Factor 4, 40kDa; Neutrophil Cytosol Factor 4; P40-Phox; NCF-4; Neutrophil Cytosolic Factor 4 (40kD); CGD3; NCF
Background

Gene Name: NCF4

NCBI Gene Entry: 4689

UniProt Entry: Q15080

Application Information

Molecular Weight: Predicted, 39 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB45

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human NCF4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Neutrophil cytosolic factor 4 antibody (Cat#2266). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Neutrophil cytosolic factor 4 antibody (Cat#2266, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HT-1080 cells with Neutrophil cytosolic factor 4 antibody(Cat#2266, 1:1,000). Nuclei were stained blue with DAPI; Neutrophil cytosolic factor 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NCF4
  • Catalog：
    2266
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Neutrophil cytosolic factor 4 antibody (Cat#2266). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Neutrophil cytosolic factor 4 antibody (Cat#2266, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with Neutrophil cytosolic factor 4 antibody(Cat#2266, 1:1,000). Nuclei were stained blue with DAPI; Neutrophil cytosolic factor 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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