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Home>Product>Primary Antibody

KD-Validated Semaphorin 4D Recombinant Rabbit mAb

#61975

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using semaphorin 4D antibody (Cat#61975). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with semaphorin 4D antibody (Cat#61975, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using semaphorin 4D antibody (Cat#61975). Semaphorin 4D expression in wild-type (WT) and semaphorin 4D (SEMA4D) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with semaphorin 4D antibody (Cat#61975, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SEMA4D
  • Catalog：
    61975
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated Semaphorin 4D Recombinant Rabbit mAb
Aliases Semaphorin 4D; CD100; C9orf164; Coll-4; SEMAJ; Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) And Short Cytoplasmic Domain, (Semaphorin) 4D; Semaphorin-4D; FLJ39737; BB18; GR3; A8; Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) And Short Cytoplasmic Domain, 4D; Chromosome 9 Open Reading Frame 164; CD100 Antigen; M-Sema G; M-Sema-G; COLL4
Background

Gene Name: SEMA4D

NCBI Gene Entry: 10507

UniProt Entry: Q92854

Application Information

Molecular Weight: Predicted, 96 kDa; observed, 120-140 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB6775

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human Semaphorin 4D / CD100
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using semaphorin 4D antibody (Cat#61975). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with semaphorin 4D antibody (Cat#61975, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using semaphorin 4D antibody (Cat#61975). Semaphorin 4D expression in wild-type (WT) and semaphorin 4D (SEMA4D) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with semaphorin 4D antibody (Cat#61975, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SEMA4D
  • Catalog：
    61975
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using semaphorin 4D antibody (Cat#61975). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with semaphorin 4D antibody (Cat#61975, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using semaphorin 4D antibody (Cat#61975). Semaphorin 4D expression in wild-type (WT) and semaphorin 4D (SEMA4D) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with semaphorin 4D antibody (Cat#61975, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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