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KO-Validated RICTOR Mouse mAb

#71300

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Knockout Validated
Western blotting analysis using RICTOR antibody (Cat#71300). RICTOR expression in wild-type (WT) and RICTOR knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RICTOR antibody (Cat#71300, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol:
    RICTOR
  • Catalog:
    71300
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KO-Validated RICTOR Mouse mAb
Aliases RICTOR; RPTOR Independent Companion Of MTOR Complex 2; Rapamycin-Insensitive Companion Of MTOR; KIAA1999; AVO3; PIA; AVO3 Homolog; Pianissimo; MGC39830; HAVO3; RPTOR Independent Companion Of MTOR, Complex 2; TORC2-Specific Protein AVO3
Background

Gene Name: RICTOR

NCBI Gene Entry: 253260

UniProt Entry: Q6R327

Application Information

Molecular Weight: Predicted, 192 kDa; observed, 220 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB1645

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human RICTOR
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using RICTOR antibody (Cat#71300). RICTOR expression in wild-type (WT) and RICTOR knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RICTOR antibody (Cat#71300, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using RICTOR antibody (Cat#71300). RICTOR expression in wild-type (WT) and RICTOR knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RICTOR antibody (Cat#71300, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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