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Home>Product>Primary Antibody

RNA Polymerase II Subunit C Recombinant Rabbit mAb

#2589

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Recombinant Antibody
Western blotting analysis using RNA polymerase II subunit C antibody (Cat#2589). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RNA polymerase II subunit C antibody (Cat#2589, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with RNA polymerase II subunit C antibody (Cat#2589, 1:1,000). Nuclei were stained blue with DAPI; RNA polymerase II subunit C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RNA polymerase II subunit C expression in HepG2 cells using RNA polymerase II subunit C antibody (Cat#2589, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RNA polymerase II subunit C.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    POLR2C
  • Catalog：
    2589
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name RNA Polymerase II Subunit C Recombinant Rabbit mAb
Aliases RNA Polymerase II Subunit C; RNA Polymerase II Subunit 3; RPB3; DNA-Directed RNA Polymerase II 33 KDa Polypeptide; DNA-Directed RNA Polymerase II Subunit RPB3; DNA-Directed RNA Polymerase II Subunit C; RNA Polymerase II Subunit B3; RPB31; RPB33; Polymerase (RNA) II (DNA Directed) Polypeptide C (33kD); Polymerase (RNA) II (DNA Directed) Polypeptide C, 33kDa; Polymerase (RNA) II Subunit C; HRPB33; HsRPB3
Background

Gene Name: POLR2C

NCBI Gene Entry: 5432

UniProt Entry: P19387

Application Information

Molecular Weight: Predicted, 31 kDa; observed, 33 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1535

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human POLR2C
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using RNA polymerase II subunit C antibody (Cat#2589). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RNA polymerase II subunit C antibody (Cat#2589, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with RNA polymerase II subunit C antibody (Cat#2589, 1:1,000). Nuclei were stained blue with DAPI; RNA polymerase II subunit C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of RNA polymerase II subunit C expression in HepG2 cells using RNA polymerase II subunit C antibody (Cat#2589, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RNA polymerase II subunit C.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    POLR2C
  • Catalog：
    2589
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using RNA polymerase II subunit C antibody (Cat#2589). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RNA polymerase II subunit C antibody (Cat#2589, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with RNA polymerase II subunit C antibody (Cat#2589, 1:1,000). Nuclei were stained blue with DAPI; RNA polymerase II subunit C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RNA polymerase II subunit C expression in HepG2 cells using RNA polymerase II subunit C antibody (Cat#2589, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RNA polymerase II subunit C.
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