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Home>Product>Primary Antibody

Basigin (Ok Blood Group) Mouse mAb

#6200

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Western blotting analysis using CD147 antibody (Cat#6200). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD147 antibody (Cat#6200, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells with CD147 antibody (Cat#6200, 1:1,000). Nuclei were stained blue with DAPI; CD147 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CD147 expression in HeLa cells using CD147 antibody (Cat#6200, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CD147.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BSG
  • Catalog：
    6200
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Basigin (Ok Blood Group) Mouse mAb
Aliases BSG; Basigin (Ok Blood Group); EMMPRIN; Tumor Cell-Derived Collagenase Stimulatory Factor; Extracellular Matrix Metalloproteinase Inducer; Basigin; EMPRIN; CD147; Leukocyte Activation Antigen M6; Collagenase Stimulatory Factor; Hepatoma-Associated Antigen; OK Blood Group Antigen; HAntibody18G; TCSF; 5F; Ok Blood Group; CD147 Antigen
Background

Gene Name: BSG

NCBI Gene Entry: 682

UniProt Entry: P35613

Application Information

Molecular Weight: Predicted, 42 kDa; observed, 35-70 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB7575

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human BSG
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CD147 antibody (Cat#6200). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD147 antibody (Cat#6200, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HeLa cells with CD147 antibody (Cat#6200, 1:1,000). Nuclei were stained blue with DAPI; CD147 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of CD147 expression in HeLa cells using CD147 antibody (Cat#6200, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CD147.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BSG
  • Catalog：
    6200
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using CD147 antibody (Cat#6200). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD147 antibody (Cat#6200, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells with CD147 antibody (Cat#6200, 1:1,000). Nuclei were stained blue with DAPI; CD147 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CD147 expression in HeLa cells using CD147 antibody (Cat#6200, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CD147.
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