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Home>Product>Primary Antibody

KD-Validated KIF23 Recombinant Rabbit mAb

#66867

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using KIF23 antibody (Cat#66867). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KIF23 antibody (Cat#66867, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using KIF23 antibody (Cat#66867). KIF23 expression in wild type (WT) and KIF23 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with KIF23 antibody (Cat#66867, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with KIF23 antibody (Cat#66867, 1:1,000). Nuclei were stained blue with DAPI; KIF23 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
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  • Gene Symbol：
    KIF23
  • Catalog：
    66867
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated KIF23 Recombinant Rabbit mAb
Aliases KIF23; Kinesin Family Member 23; MKLP1; MKLP-1; KNSL5; Kinesin-Like 5 (Mitotic Kinesin-Like Protein 1); Kinesin-Like Protein KIF23; Congenital Dyserythropoietic Anemia, Type III; Mitotic Kinesin-Like Protein 1; Kinesin-Like Protein 5; CDAIII; CDAN3A; CDAN3; CDA3; CHO1
Background

Gene Name: KIF23

NCBI Gene Entry: 9493

UniProt Entry: Q02241

Application Information

Molecular Weight: Predicted, 110 kDa; observed, 100-120 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB1085

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human KIF23
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using KIF23 antibody (Cat#66867). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KIF23 antibody (Cat#66867, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using KIF23 antibody (Cat#66867). KIF23 expression in wild type (WT) and KIF23 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with KIF23 antibody (Cat#66867, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with KIF23 antibody (Cat#66867, 1:1,000). Nuclei were stained blue with DAPI; KIF23 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    KIF23
  • Catalog：
    66867
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using KIF23 antibody (Cat#66867). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KIF23 antibody (Cat#66867, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using KIF23 antibody (Cat#66867). KIF23 expression in wild type (WT) and KIF23 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with KIF23 antibody (Cat#66867, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with KIF23 antibody (Cat#66867, 1:1,000). Nuclei were stained blue with DAPI; KIF23 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
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