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Glutamic-Oxaloacetic Transaminase 2 Recombinant Rabbit mAb

#3918

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Recombinant Antibody
Western blotting analysis using glutamic-oxaloacetic transaminase 2 antibody (Cat#3918). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with glutamic-oxaloacetic transaminase 2 antibody (Cat#3918, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded mouse brain using glutamic-oxaloacetic transaminase 2 antibody (Cat#3918, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of glutamic-oxaloacetic transaminase 2 expression in HepG2 cells using glutamic-oxaloacetic transaminase 2 antibody (Cat#3918, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, glutamic-oxaloacetic transaminase 2.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    GOT2
  • Catalog:
    3918
  • Application:
    WB, FCM, IHC-P
  • Species Reactivity:
    H, M, R
Information
Product Name Glutamic-Oxaloacetic Transaminase 2 Recombinant Rabbit mAb
Aliases GOT2; Glutamic-Oxaloacetic Transaminase 2; KYAT4; Kynurenine Aminotransferase IV; MAspAT; KATIV; KAT4; Plasma Membrane-Associated Fatty Acid-Binding Protein; Glutamic-Oxaloacetic Transaminase 2, Mitochondrial; Aspartate Aminotransferase, Mitochondrial; Kynurenine--Oxoglutarate Transaminase IV; Kynurenine--Oxoglutarate Transaminase 4; Glutamate Oxaloacetate Transaminase 2; Kynurenine Aminotransferase 4; Aspartate Aminotransferase 2; Fatty Acid-Binding Protein; Aspartate Transaminase 2; Transaminase A; EC 2.6.1.1; MitAAT; FABP-1; FABPpm; Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2); EC 2.6.1.7; EC 2.6.1; MITAAT; DEE82
Background

Gene Name: GOT2

NCBI Gene Entry: 2806

UniProt Entry: P00505

Application Information

Molecular Weight: Predicted, 48 kDa; observed, 43 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB8680

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)

Immunogen A synthesized peptide derived from human GOT2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using glutamic-oxaloacetic transaminase 2 antibody (Cat#3918). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with glutamic-oxaloacetic transaminase 2 antibody (Cat#3918, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded mouse brain using glutamic-oxaloacetic transaminase 2 antibody (Cat#3918, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of glutamic-oxaloacetic transaminase 2 expression in HepG2 cells using glutamic-oxaloacetic transaminase 2 antibody (Cat#3918, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, glutamic-oxaloacetic transaminase 2.
Western blotting analysis using glutamic-oxaloacetic transaminase 2 antibody (Cat#3918). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with glutamic-oxaloacetic transaminase 2 antibody (Cat#3918, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded mouse brain using glutamic-oxaloacetic transaminase 2 antibody (Cat#3918, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of glutamic-oxaloacetic transaminase 2 expression in HepG2 cells using glutamic-oxaloacetic transaminase 2 antibody (Cat#3918, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, glutamic-oxaloacetic transaminase 2.
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