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Home>Product>Primary Antibody

KD-Validated BRAT1 Recombinant Rabbit mAb

#62000

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using BRAT1 antibody (Cat#62000). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BRAT1 antibody (Cat#62000, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using BRAT1 antibody (Cat#62000). BRAT1 expression in wild-type (WT) and BRAT1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BRAT1 antibody (Cat#62000, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BRAT1
  • Catalog：
    62000
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated BRAT1 Recombinant Rabbit mAb
Aliases BRAT1; BRCA1 Associated ATM Activator 1; BRCA1-Associated Protein Required For ATM Activation Protein 1; BRCA1-Associated ATM Activator 1; BAAT1; MGC22916; C7orf27; HEAT Repeat-Containing Protein C7orf27; Chromosome 7 Open Reading Frame 27; C7ORF27; NEDCAS; RMFSL
Background

Gene Name: BRAT1

NCBI Gene Entry: 221927

UniProt Entry: Q6PJG6

Application Information

Molecular Weight: Predicted, 88 kDa; observed, 74 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB7805

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human BRAT1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using BRAT1 antibody (Cat#62000). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BRAT1 antibody (Cat#62000, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using BRAT1 antibody (Cat#62000). BRAT1 expression in wild-type (WT) and BRAT1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BRAT1 antibody (Cat#62000, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BRAT1
  • Catalog：
    62000
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using BRAT1 antibody (Cat#62000). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BRAT1 antibody (Cat#62000, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using BRAT1 antibody (Cat#62000). BRAT1 expression in wild-type (WT) and BRAT1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BRAT1 antibody (Cat#62000, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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