Western blotting analysis using BMAL1 antibody (Cat#6202). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BMAL1 antibody (Cat#6202, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with BMAL1 antibody (Cat#6202, 1:1,000). Nuclei were stained blue with DAPI; BMAL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of BMAL1 expression in C2C12 cells using BMAL1 antibody (Cat#6202, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BMAL1.
BMAL1; Basic Helix-Loop-Helix ARNT Like 1; BHLHe5; PASD3; MOP3; Brain And Muscle ARNT-Like 1; ARNTL1; ARNTL; JAP3; Aryl Hydrocarbon Receptor Nuclear Translocator-Like Protein 1; Aryl Hydrocarbon Receptor Nuclear Translocator Like; Basic Helix-Loop-Helix ARNT-Like Protein 1; Class E Basic Helix-Loop-Helix Protein 5; Basic Helix-Loop-Helix Family Member E5; Basic-Helix-Loop-Helix-PAS Protein MOP3; PAS Domain-Containing Protein 3; Member Of PAS Superfamily 3; PAS Domain Containing 3; Member Of PAS Protein 3; BHLH-PAS Protein JAP3; Mutant Basic Helix-Loop-Helix ARNT-Like Protein 1; Testis Tissue Sperm-Binding Protein Li 50e; Basic-Helix-Loop-Helix-PAS Orphan MOP3; ARNT-Like Protein 1, Brain And Muscle; BMAL1c; BHLHE5; TIC
Western blotting analysis using BMAL1 antibody (Cat#6202). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BMAL1 antibody (Cat#6202, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with BMAL1 antibody (Cat#6202, 1:1,000). Nuclei were stained blue with DAPI; BMAL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of BMAL1 expression in C2C12 cells using BMAL1 antibody (Cat#6202, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BMAL1.
Western blotting analysis using BMAL1 antibody (Cat#6202). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BMAL1 antibody (Cat#6202, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with BMAL1 antibody (Cat#6202, 1:1,000). Nuclei were stained blue with DAPI; BMAL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of BMAL1 expression in C2C12 cells using BMAL1 antibody (Cat#6202, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BMAL1.
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