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Home>Product>Primary Antibody

KD-Validated MAD2L1BP Mouse mAb

#65917

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shRNA Knockdown Validated
Western blotting analysis using MAD2L1BP antibody (Cat#65917). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAD2L1BP antibody (Cat#65917, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using MAD2L1BP antibody (Cat#65917). MAD2L1BP expression in wild-type (WT) and MAD2L1BP shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAD2L1BP antibody (Cat#65917, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of MAD2L1BP expression in HAP-1 cells using MAD2L1BP antibody (Cat#65917, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MAD2L1BP.
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Unit price：
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  • Gene Symbol：
    MAD2L1BP
  • Catalog：
    65917
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated MAD2L1BP Mouse mAb
Aliases MAD2L1BP; MAD2L1 Binding Protein; CMT2; KIAA0110; P31comet; MAD2L1-Binding Protein; Caught By MAD2 Protein; DJ261G23.1; P31(Comet)
Background

Gene Name: MAD2L1BP

NCBI Gene Entry: 9587

UniProt Entry: Q15013

Application Information

Molecular Weight: Predicted, 31 kDa; observed, 36 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5820

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human MAD2L1BP
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using MAD2L1BP antibody (Cat#65917). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAD2L1BP antibody (Cat#65917, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using MAD2L1BP antibody (Cat#65917). MAD2L1BP expression in wild-type (WT) and MAD2L1BP shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAD2L1BP antibody (Cat#65917, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of MAD2L1BP expression in HAP-1 cells using MAD2L1BP antibody (Cat#65917, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MAD2L1BP.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAD2L1BP
  • Catalog：
    65917
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using MAD2L1BP antibody (Cat#65917). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAD2L1BP antibody (Cat#65917, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using MAD2L1BP antibody (Cat#65917). MAD2L1BP expression in wild-type (WT) and MAD2L1BP shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAD2L1BP antibody (Cat#65917, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of MAD2L1BP expression in HAP-1 cells using MAD2L1BP antibody (Cat#65917, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MAD2L1BP.
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