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Home>Product>Primary Antibody

ITPR1 Recombinant Rabbit mAb

#5530

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Recombinant Antibody
Western blotting analysis using ITPR1 antibody (Cat#5530). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ITPR1 antibody (Cat#5530, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9C2 cells with ITPR1 antibody (Cat#5530, 1:1,000). Nuclei were stained blue with DAPI; ITPR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ITPR1 expression in H9c2 cells using ITPR1 antibody (Cat#5530, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ITPR1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ITPR1
  • Catalog：
    5530
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name ITPR1 Recombinant Rabbit mAb
Aliases ITPR1; Inositol 1,4,5-Trisphosphate Receptor Type 1; Insp3r1; PPP1R94; IP3R1; ACV; Inositol 1,4,5-Trisphosphate-Gated Calcium Channel ITPR1; Protein Phosphatase 1, Regulatory Subunit 94; Type 1 Inositol 1,4,5-Trisphosphate Receptor; Inositol 1,4,5 Trisphosphate Receptor; Type 1 InsP3 Receptor; IP3R 1; SCA15; SCA16; SCA29; Inositol 1,4,5-Trisphosphate Receptor, Type 1; Inositol 1,4,5-Triphosphate Receptor, Type 1; Spinocerebellar Ataxia 15; Spinocerebellar Ataxia 16; Spinocerebellar Ataxia 29; IP3 Receptor Isoform 1; IP3 Receptor; INSP3R1; InsP3R1; CLA4; IP3R
Background

Gene Name: ITPR1

NCBI Gene Entry: 3708

UniProt Entry: Q14643

Application Information

Molecular Weight: Predicted, 314 kDa; observed, 314 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1930

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human ITPR1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ITPR1 antibody (Cat#5530). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ITPR1 antibody (Cat#5530, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of H9C2 cells with ITPR1 antibody (Cat#5530, 1:1,000). Nuclei were stained blue with DAPI; ITPR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ITPR1 expression in H9c2 cells using ITPR1 antibody (Cat#5530, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ITPR1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ITPR1
  • Catalog：
    5530
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ITPR1 antibody (Cat#5530). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ITPR1 antibody (Cat#5530, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9C2 cells with ITPR1 antibody (Cat#5530, 1:1,000). Nuclei were stained blue with DAPI; ITPR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ITPR1 expression in H9c2 cells using ITPR1 antibody (Cat#5530, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ITPR1.
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