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Home>Product>Primary Antibody

KD-Validated ALKBH2 Recombinant Rabbit mAb

#61804

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Recombinant Antibody
Knockdown Validated
Western blotting analysis using ALKBH2 antibody (Cat#61804). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALKBH2 antibody (Cat#61804, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ALKBH2 antibody (Cat#61804). ALKBH2 expression in wild-type (WT) and ALKBH2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ALKBH2 antibody (Cat#61804, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ALKBH2 expression in HepG2 cells using ALKBH2 antibody (Cat#61804, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ALKBH2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ALKBH2
  • Catalog：
    61804
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated ALKBH2 Recombinant Rabbit mAb
Aliases ALKBH2; AlkB Homolog 2, Alpha-Ketoglutarate Dependent Dioxygenase; ABH2; Alpha-Ketoglutarate-Dependent Dioxygenase AlkB Homolog 2; Alkylated DNA Repair Protein AlkB Homolog 2; DNA Oxidative Demethylase ALKBH2; MGC90512; Oxy DC1; AlkB, Alkylation Repair Homolog 2 (E. Coli); AlkB, Alkylation Repair Homolog 2; 2OG-Fe(II) Oxy DC1; EC 1.14.11.33
Background

Gene Name: ALKBH2

NCBI Gene Entry: 121642

UniProt Entry: Q6NS38

Application Information

Molecular Weight: Predicted, 29 kDa; observed, 25 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB3840

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human ALKBH2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using ALKBH2 antibody (Cat#61804). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALKBH2 antibody (Cat#61804, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using ALKBH2 antibody (Cat#61804). ALKBH2 expression in wild-type (WT) and ALKBH2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ALKBH2 antibody (Cat#61804, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of ALKBH2 expression in HepG2 cells using ALKBH2 antibody (Cat#61804, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ALKBH2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ALKBH2
  • Catalog：
    61804
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ALKBH2 antibody (Cat#61804). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALKBH2 antibody (Cat#61804, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ALKBH2 antibody (Cat#61804). ALKBH2 expression in wild-type (WT) and ALKBH2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ALKBH2 antibody (Cat#61804, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ALKBH2 expression in HepG2 cells using ALKBH2 antibody (Cat#61804, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ALKBH2.
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