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Home>Product>Primary Antibody

KD-Validated SUMO1 Mouse mAb

#63746

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shRNA Knockdown Validated
Western blotting analysis using small ubiquitin like modifier 1 antibody (Cat#63746). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with small ubiquitin like modifier 1 antibody (Cat#63746, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using small ubiquitin like modifier 1 antibody (Cat#63746). Small ubiquitin like modifier 1 expression in wild-type (WT) and small ubiquitin like modifier 1 (SUMO1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with small ubiquitin like modifier 1 antibody (Cat#63746, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using small ubiquitin like modifier 1 antibody (Cat#63746, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using small ubiquitin like modifier 1 antibody (Cat#63746, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Small ubiquitin like modifier 1 expression in HAP-1 cells using Small ubiquitin like modifier 1 antibody (Cat#63746, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Small ubiquitin like modifier 1.
Validation of Small ubiquitin like modifier 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Small ubiquitin like modifier 1 antibody (Cat#63746, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    SUMO1
  • Catalog：
    63746
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated SUMO1 Mouse mAb
Aliases SUMO1; Small Ubiquitin Like Modifier 1; SMT3H3; SMT3C; GMP1; SUMO-1; OFC10; PIC1; UBL1; Ubiquitin-Homology Domain Protein PIC1; Small Ubiquitin-Related Modifier 1; Ubiquitin-Like Protein SMT3C; Ubiquitin-Like Protein UBL1; SMT3 Homolog 3; Sentrin; SMT3 Suppressor Of Mif Two 3 Homolog 1 (S. Cerevisiae); SMT3 Suppressor Of Mif Two 3 Homolog 1 (Yeast); SMT3 Suppressor Of Mif Two 3 Homolog 1; Ubiquitin-Like 1 (Sentrin); GAP Modifying Protein 1; GAP-Modifying Protein 1; SENP2; Smt3C; DAP1; SMT3
Background

Gene Name: SUMO1

NCBI Gene Entry: 7341

UniProt Entry: P63165

Application Information

Molecular Weight: Predicted, 12 kDa; observed, 75 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB7175

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)
Immunogen Recombinant protein of human SUMO1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using small ubiquitin like modifier 1 antibody (Cat#63746). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with small ubiquitin like modifier 1 antibody (Cat#63746, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using small ubiquitin like modifier 1 antibody (Cat#63746). Small ubiquitin like modifier 1 expression in wild-type (WT) and small ubiquitin like modifier 1 (SUMO1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with small ubiquitin like modifier 1 antibody (Cat#63746, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using small ubiquitin like modifier 1 antibody (Cat#63746, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse kidney using small ubiquitin like modifier 1 antibody (Cat#63746, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of Small ubiquitin like modifier 1 expression in HAP-1 cells using Small ubiquitin like modifier 1 antibody (Cat#63746, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Small ubiquitin like modifier 1.
  • Validation of Small ubiquitin like modifier 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Small ubiquitin like modifier 1 antibody (Cat#63746, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SUMO1
  • Catalog：
    63746
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using small ubiquitin like modifier 1 antibody (Cat#63746). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with small ubiquitin like modifier 1 antibody (Cat#63746, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using small ubiquitin like modifier 1 antibody (Cat#63746). Small ubiquitin like modifier 1 expression in wild-type (WT) and small ubiquitin like modifier 1 (SUMO1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with small ubiquitin like modifier 1 antibody (Cat#63746, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using small ubiquitin like modifier 1 antibody (Cat#63746, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using small ubiquitin like modifier 1 antibody (Cat#63746, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Small ubiquitin like modifier 1 expression in HAP-1 cells using Small ubiquitin like modifier 1 antibody (Cat#63746, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Small ubiquitin like modifier 1.
Validation of Small ubiquitin like modifier 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Small ubiquitin like modifier 1 antibody (Cat#63746, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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