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Home>Product>Primary Antibody

G Protein Subunit Beta 2 Recombinant Rabbit mAb

#4634

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Recombinant Antibody
Western blotting analysis using G protein subunit beta 2 antibody (Cat#4634). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with G protein subunit beta 2 antibody (Cat#4634, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with G protein subunit beta 2 antibody (Cat#4634, 1:1,000). Nuclei were stained blue with DAPI; G protein subunit beta 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of G protein subunit beta 2 expression in HT-1080 cells using G protein subunit beta 2 antibody (Cat#4634, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, G protein subunit beta 2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    GNB2
  • Catalog：
    4634
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name G Protein Subunit Beta 2 Recombinant Rabbit mAb
Aliases GNB2; Transducin Beta Chain 2; Signal-Transducing Guanine Nucleotide-Binding Regulatory Protein Beta Subunit 2; Guanine Nucleotide Binding Protein (G Protein), Beta Polypeptide 2; Guanine Nucleotide-Binding Protein G(I)/G(S)/G(T) Beta Subunit 2; Guanine Nucleotide-Binding Protein G(I)/G(S)/G(T) Subunit Beta-2; G Protein, Beta-2 Subunit; G Protein Subunit Beta-2; Heterotrimeric Guanine Nucleotide-Binding Protein 2C1; Epididymis Secretory Sperm Binding Protein; SSS4; NEDHYDF; HG2C1;SSS4
Background

Gene Name: GNB2

NCBI Gene Entry: 2783

UniProt Entry: P62879

Application Information

Molecular Weight: Predicted, 37 kDa; observed, 35 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12180

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human GNB2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using G protein subunit beta 2 antibody (Cat#4634). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with G protein subunit beta 2 antibody (Cat#4634, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HT-1080 cells with G protein subunit beta 2 antibody (Cat#4634, 1:1,000). Nuclei were stained blue with DAPI; G protein subunit beta 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of G protein subunit beta 2 expression in HT-1080 cells using G protein subunit beta 2 antibody (Cat#4634, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, G protein subunit beta 2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    GNB2
  • Catalog：
    4634
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using G protein subunit beta 2 antibody (Cat#4634). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with G protein subunit beta 2 antibody (Cat#4634, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with G protein subunit beta 2 antibody (Cat#4634, 1:1,000). Nuclei were stained blue with DAPI; G protein subunit beta 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of G protein subunit beta 2 expression in HT-1080 cells using G protein subunit beta 2 antibody (Cat#4634, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, G protein subunit beta 2.
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