Western blotting analysis using Phospho-STAT3 (S727) antibody (Cat#1689). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-STAT3 (S727) antibody (Cat#1689, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Phospho-STAT3 (S727) antibody (Cat#1689, 1:1,000). Nuclei were stained blue with DAPI; Phospho-STAT3 (S727) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-STAT3 (S727) expression in HepG2 cells using Phospho-STAT3 (S727) antibody (Cat#1689, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-STAT3 (S727).
STAT3; Signal Transducer And Activator Of Transcription 3; APRF; Acute-Phase Response Factor; Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor); DNA-Binding Protein APRF; ADMIO1; ADMIO; HIES
Western blotting analysis using Phospho-STAT3 (S727) antibody (Cat#1689). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-STAT3 (S727) antibody (Cat#1689, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Phospho-STAT3 (S727) antibody (Cat#1689, 1:1,000). Nuclei were stained blue with DAPI; Phospho-STAT3 (S727) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-STAT3 (S727) expression in HepG2 cells using Phospho-STAT3 (S727) antibody (Cat#1689, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-STAT3 (S727).
Western blotting analysis using Phospho-STAT3 (S727) antibody (Cat#1689). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-STAT3 (S727) antibody (Cat#1689, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Phospho-STAT3 (S727) antibody (Cat#1689, 1:1,000). Nuclei were stained blue with DAPI; Phospho-STAT3 (S727) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-STAT3 (S727) expression in HepG2 cells using Phospho-STAT3 (S727) antibody (Cat#1689, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-STAT3 (S727).
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