KD-Validated EFEMP1 Recombinant Rabbit mAb - Product

KD-Validated EFEMP1 Recombinant Rabbit mAb

#65222

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Recombinant Antibody
shRNA Knockdown Validated

Western blotting analysis using EFEMP1 antibody (Cat#65222). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EFEMP1 antibody (Cat#65222, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using EFEMP1 antibody (Cat#65222). EFEMP1 expression in wild-type (WT) and EFEMP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with EFEMP1 antibody (Cat#65222, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of EFEMP1 expression in HeLa cells using EFEMP1 antibody (Cat#65222, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, EFEMP1.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

EFEMP1
Catalog：

65222
Application：

WB, FCM
Species Reactivity：

H

Information

Data

Information

Product Name	KD-Validated EFEMP1 Recombinant Rabbit mAb
Aliases	EFEMP1; EGF Containing Fibulin Extracellular Matrix Protein 1; FBLN3; EGF-Containing Fibulin-Like Extracellular Matrix Protein 1; S1-5; MTLV; FBNL; EGF Containing Fibulin Like Extracellular Matrix Protein 1; Extracellular Protein S1-5; Fibulin-3; FIBL-3; DHRD; Glaucoma 1, Open Angle, H (Adult-Onset); Fibrillin-Like Protein; Fibrillin-Like; Fibulin 3; ARCL1D; GLC1H; DRAD; MLVT
Background	Gene Name: EFEMP1 NCBI Gene Entry: 2202 UniProt Entry: Q12805
Application Information	Molecular Weight: Predicted, 55 kDa; observed, 55-65 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB230 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human EFEMP1/Fibulin-3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using EFEMP1 antibody (Cat#65222). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EFEMP1 antibody (Cat#65222, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using EFEMP1 antibody (Cat#65222). EFEMP1 expression in wild-type (WT) and EFEMP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with EFEMP1 antibody (Cat#65222, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of EFEMP1 expression in HeLa cells using EFEMP1 antibody (Cat#65222, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, EFEMP1.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

EFEMP1
Catalog：

65222
Application：

WB, FCM
Species Reactivity：

H

Datasheet

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