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Home>Product>Primary Antibody

MAGEC2 Recombinant Rabbit mAb

#5557

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Recombinant Antibody
Western blotting analysis using MAGEC2 antibody (Cat#5557). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAGEC2 antibody (Cat#5557, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with MAGEC2 antibody (Cat#5557,1:1,000). Nuclei were stained blue with DAPI; MAGEC2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAGEC2
  • Catalog：
    5557
  • Application：
    WB, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name MAGEC2 Recombinant Rabbit mAb
Aliases MAGEC2; MAGE Family Member C2; HCA587; CT10; Cancer/Testis Antigen 10; MAGEE1; Melanoma Antigen, Family E, 1, Cancer/Testis Specific; Hepatocellular Carcinoma-Associated Antigen 587; Melanoma-Associated Antigen C2; Melanoma Antigen Family C2; MAGE-C2 Antigen; MAGE-E1 Antigen; MAGE-C2; Hepatocellular Cancer Antigen 587; Melanoma Antigen Family C, 2
Background

Gene Name: MAGEC2

NCBI Gene Entry: 51438

UniProt Entry: Q9UBF1

Application Information

Molecular Weight: Predicted, 41 kDa; observed, 55 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4245

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human MAGEC2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using MAGEC2 antibody (Cat#5557). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAGEC2 antibody (Cat#5557, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HT-1080 cells with MAGEC2 antibody (Cat#5557,1:1,000). Nuclei were stained blue with DAPI; MAGEC2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAGEC2
  • Catalog：
    5557
  • Application：
    WB, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using MAGEC2 antibody (Cat#5557). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAGEC2 antibody (Cat#5557, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with MAGEC2 antibody (Cat#5557,1:1,000). Nuclei were stained blue with DAPI; MAGEC2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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