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Home>Product>Primary Antibody

GCNT1 Mouse mAb

#6277

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Western blotting analysis using GCNT1 antibody (Cat#6277). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GCNT1 antibody (Cat#6277, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with GCNT1 antibody (Cat#6277,1:1,000). Nuclei were stained blue with DAPI; GCNT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of GCNT1 expression in HAP-1 cells using GCNT1 antibody (Cat#6277,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, GCNT1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    GCNT1
  • Catalog：
    6277
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name GCNT1 Mouse mAb
Aliases GCNT1; Glucosaminyl (N-Acetyl) Transferase 1; C2GNT; NAGCT2; NACGT2; Glucosaminyl (N-Acetyl) Transferase 1, Core 2 (Beta-1,6-N-Acetylglucosaminyltransferase); Beta-1,3-Galactosyl-O-Glycosyl-Glycoprotein Beta-1,6-N-Acetylglucosaminyltransferase; Leukocyte Type Core 2 Beta-1,6-N-Acetylglucosaminyltransferase; Core 2 Beta1,6 N-Acetylglucosaminyltransferase-I; Core2-GlcNAc-Transferase; C2GlcNAcT; Beta-1,3-Galactosyl-O-Glycosyl-Glycoprotein Beta-1,6-N--Acetylglucosaminyltransferase; Core 2 Beta-1,6-N-Acetylglucosaminyltransferase I; Core 2 Beta-1,6-N-Acetylglucosaminyltransferase; Glucosaminyl (N-Acetyl) Transferase 1, Core 2; Beta-1,6-N-Acetylglucosaminyltransferase; Core 2 Branching Enzyme; Core 2-Branching Enzyme; EC 2.4.1.102; Core 2 GnT; Core 2 GNT; C2GNT-L; C2GnT-L; C2GNT1; G6NT
Background

Gene Name: GCNT1

NCBI Gene Entry: 2650

UniProt Entry: Q02742

Application Information

Molecular Weight: Predicted, 50 kDa; observed, 50 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB8315

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human GCNT1
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using GCNT1 antibody (Cat#6277). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GCNT1 antibody (Cat#6277, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with GCNT1 antibody (Cat#6277,1:1,000). Nuclei were stained blue with DAPI; GCNT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of GCNT1 expression in HAP-1 cells using GCNT1 antibody (Cat#6277,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, GCNT1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    GCNT1
  • Catalog：
    6277
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using GCNT1 antibody (Cat#6277). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GCNT1 antibody (Cat#6277, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with GCNT1 antibody (Cat#6277,1:1,000). Nuclei were stained blue with DAPI; GCNT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of GCNT1 expression in HAP-1 cells using GCNT1 antibody (Cat#6277,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, GCNT1.
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