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Home>Product>Primary Antibody

KD-Validated PDCD4 Recombinant Rabbit mAb

#62028

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Recombinant Antibody
Knockdown Validated
Western blotting analysis using PDCD4 antibody (Cat#62028). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDCD4 antibody (Cat#62028, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PDCD4 antibody (Cat#62028). PDCD4 expression in wild-type (WT) and PDCD4 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PDCD4 antibody (Cat#62028, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PDCD4
  • Catalog：
    62028
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated PDCD4 Recombinant Rabbit mAb
Aliases PDCD4; Programmed Cell Death 4; H731; Programmed Cell Death 4 (Neoplastic Transformation Inhibitor); Neoplastic Transformation Inhibitor Protein; Programmed Cell Death Protein 4; Nuclear Antigen H731; Protein 197/15a; Nuclear Antigen H731-Like
Background

Gene Name: PDCD4

NCBI Gene Entry: 27250

UniProt Entry: Q53EL6

Application Information

Molecular Weight: Predicted, 52 kDa; observed, 55 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB7880

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human PDCD4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using PDCD4 antibody (Cat#62028). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDCD4 antibody (Cat#62028, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using PDCD4 antibody (Cat#62028). PDCD4 expression in wild-type (WT) and PDCD4 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PDCD4 antibody (Cat#62028, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PDCD4
  • Catalog：
    62028
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using PDCD4 antibody (Cat#62028). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDCD4 antibody (Cat#62028, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PDCD4 antibody (Cat#62028). PDCD4 expression in wild-type (WT) and PDCD4 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PDCD4 antibody (Cat#62028, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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