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  • Primary Antibody
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Home>Product>Primary Antibody

EIF4E Mouse mAb

#6496

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Western blotting analysis using EIF4E antibody (Cat#6496). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF4E antibody (Cat#6496, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with EIF4E antibody (Cat#6496, 1:1,000). Nuclei were stained blue with DAPI; EIF4E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of EIF4E expression in HAP-1 cells using EIF4E antibody (Cat#6496, 1:2,000). Green, isotype control; red, EIF4E.
Please select the specifications of the product.
Specification：
20 μL，100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    EIF4E
  • Catalog：
    6496
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name EIF4E Mouse mAb
Aliases EIF4E; Eukaryotic Translation Initiation Factor 4E; EIF4EL1; EIF4E1; EIF4F; MRNA Cap-Binding Protein; EIF-4F 25 KDa Subunit; EIF-4E; Eukaryotic Translation Initiation Factor 4E-Like 1; AUTS19; EIF4E; CBP
Background

Gene Name: EIF4E

NCBI Gene Entry: 1977

UniProt Entry: P06730

Application Information

Molecular Weight: Predicted, 25 kDa; observed, 25 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9590

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human EIF4E
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using EIF4E antibody (Cat#6496). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF4E antibody (Cat#6496, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with EIF4E antibody (Cat#6496, 1:1,000). Nuclei were stained blue with DAPI; EIF4E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Flow cytometric analysis of EIF4E expression in HAP-1 cells using EIF4E antibody (Cat#6496, 1:2,000). Green, isotype control; red, EIF4E.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    EIF4E
  • Catalog：
    6496
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using EIF4E antibody (Cat#6496). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF4E antibody (Cat#6496, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with EIF4E antibody (Cat#6496, 1:1,000). Nuclei were stained blue with DAPI; EIF4E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of EIF4E expression in HAP-1 cells using EIF4E antibody (Cat#6496, 1:2,000). Green, isotype control; red, EIF4E.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
  154. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_cart.php ( 3.88 KB )
  155. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/foot.php ( 5.05 KB )
  156. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  157. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/response/Html.php ( 0.97 KB )
  158. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.003018s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.003226s ]
  3. SELECT * FROM `goods` WHERE `code` = '6496' LIMIT 1 [ RunTime:0.002846s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001152s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002100s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001325s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 10333 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001629s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.002623s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 10333 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000976s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.001270s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.001967s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.239262s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5750,5254,6379,8406,8178,4488,10250,10125,4180,7292) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001284s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.001611s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000561s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000853s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000606s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000703s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000537s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000288s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000307s ]
0.363568s