Western blotting analysis using chloride intracellular channel 4 antibody (Cat#4428). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with chloride intracellular channel 4 antibody (Cat#4428, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of chloride intracellular channel 4 expression in HAP-1 cells using chloride intracellular channel 4 antibody (Cat#4428, 1:2,000). Green, isotype control; red, chloride intracellular channel 4.
Immunocytochemical staining of HAP-1 cells with Chloride intracellular channel 4 antibody (Cat#4428, 1:1,000). Nuclei were stained blue with DAPI; Chloride intracellular channel 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using chloride intracellular channel 4 antibody (Cat#4428). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with chloride intracellular channel 4 antibody (Cat#4428, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of chloride intracellular channel 4 expression in HAP-1 cells using chloride intracellular channel 4 antibody (Cat#4428, 1:2,000). Green, isotype control; red, chloride intracellular channel 4.
Immunocytochemical staining of HAP-1 cells with Chloride intracellular channel 4 antibody (Cat#4428, 1:1,000). Nuclei were stained blue with DAPI; Chloride intracellular channel 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using chloride intracellular channel 4 antibody (Cat#4428). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with chloride intracellular channel 4 antibody (Cat#4428, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of chloride intracellular channel 4 expression in HAP-1 cells using chloride intracellular channel 4 antibody (Cat#4428, 1:2,000). Green, isotype control; red, chloride intracellular channel 4.
Immunocytochemical staining of HAP-1 cells with Chloride intracellular channel 4 antibody (Cat#4428, 1:1,000). Nuclei were stained blue with DAPI; Chloride intracellular channel 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000903s ]
SELECT * FROM `goods` WHERE `code` = '4428' LIMIT 1 [ RunTime:0.000781s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000512s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000460s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000447s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5750 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000414s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000460s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5750 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000282s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000429s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000301s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.053899s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5142,8662,3999,10180,6589,7765,10403,6712,4069,8831) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001057s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000682s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000302s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000786s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000355s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000413s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000499s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000611s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000317s ]
