Western blotting analysis using Hsp27 antibody (Cat#5525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsp27 antibody (Cat#5525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HACAT cells with Hsp27 antibody (Cat#5525, 1:1,000) . Nuclei were stained blue with DAPI; Hsp27 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of Hsp27 expression in HaCaT cells using Hsp27 antibody (Cat#5525,1:2,000). Green, isotype control; red, Hsp27.
HSPB1; Heat Shock Protein Family B (Small) Member 1; HSP27; HSP28; Hs.76067; Hsp25; CMT2F; Heat Shock Protein Family B Member 1; Estrogen-Regulated 24 KDa Protein; Stress-Responsive Protein 27; Heat Shock 27kDa Protein 1; Heat Shock 27kD Protein 1; Heat Shock Protein Beta-1; 28 KDa Heat Shock Protein; Heat Shock 27 KDa Protein; SRP27; Epididymis Secretory Protein Li 102; HEL-S-102; HSP 27; HMN2B; HMND3; HspB1
Western blotting analysis using Hsp27 antibody (Cat#5525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsp27 antibody (Cat#5525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HACAT cells with Hsp27 antibody (Cat#5525, 1:1,000) . Nuclei were stained blue with DAPI; Hsp27 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of Hsp27 expression in HaCaT cells using Hsp27 antibody (Cat#5525,1:2,000). Green, isotype control; red, Hsp27.
Western blotting analysis using Hsp27 antibody (Cat#5525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsp27 antibody (Cat#5525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HACAT cells with Hsp27 antibody (Cat#5525, 1:1,000) . Nuclei were stained blue with DAPI; Hsp27 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of Hsp27 expression in HaCaT cells using Hsp27 antibody (Cat#5525,1:2,000). Green, isotype control; red, Hsp27.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000668s ]
SELECT * FROM `goods` WHERE `code` = '5525' LIMIT 1 [ RunTime:0.000457s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000563s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000345s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000381s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6589 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000336s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000480s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6589 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000278s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000363s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000230s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.054534s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5751,7839,7519,3920,5311,8368,6801,6972,8474,7925) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000844s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000489s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000205s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000455s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000227s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000303s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000440s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000254s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000208s ]
