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Home>Product>Primary Antibody

KD-Validated Histone H3 (acetyl K27) Mouse mAb

#62052

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shRNA Knockdown Validated
Western blotting analysis using histone H3 (acetyl K27) antibody (Cat#62052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone H3 (acetyl K27) antibody (Cat#62052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using histone H3 (acetyl K27) antibody (Cat#62052). Histone H3 (acetyl K28) expression in wild-type (WT) and H3C1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with histone H3 (acetyl K27) antibody (Cat#62052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    H3C1
  • Catalog：
    62052
  • Application：
    WB
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated Histone H3 (acetyl K27) Mouse mAb
Aliases H3C1; H3 Clustered Histone 1; HIST1H3A; H3/A; H3FA; Histone Cluster 1 H3 Family Member A; H3 Histone Family, Member A; Histone Cluster 1, H3a; Histone 1, H3a; Histone H3.1; Histone H3/A; H3FC HIST1H3C; Histone H3/B; Histone H3/C; Histone H3/D; Histone H3/F; Histone H3/H; Histone H3/I; Histone; H3/J; Histone H3/K; Histone H3/L; HIST1H3B; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FL; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ
Background

Gene Name: H3C1

NCBI Gene Entry: 8350

UniProt Entry: P68431

Application Information

Molecular Weight: Predicted, 15 kDa; observed, 17 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9325

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human Histone H3 (acetyl K27)
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Data
  • Western blotting analysis using histone H3 (acetyl K27) antibody (Cat#62052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone H3 (acetyl K27) antibody (Cat#62052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using histone H3 (acetyl K27) antibody (Cat#62052). Histone H3 (acetyl K28) expression in wild-type (WT) and H3C1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with histone H3 (acetyl K27) antibody (Cat#62052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    H3C1
  • Catalog：
    62052
  • Application：
    WB
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using histone H3 (acetyl K27) antibody (Cat#62052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone H3 (acetyl K27) antibody (Cat#62052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using histone H3 (acetyl K27) antibody (Cat#62052). Histone H3 (acetyl K28) expression in wild-type (WT) and H3C1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with histone H3 (acetyl K27) antibody (Cat#62052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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