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Home>Product>Primary Antibody

TFAM Mouse mAb

#6448

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Western blotting analysis using TFAM antibody (Cat#6448). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TFAM antibody (Cat#6448, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with TFAM antibody (Cat#6448, 1:1,000). Nuclei were stained blue with DAPI; TFAM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TFAM expression in HAP-1 cells using TFAM antibody (Cat#6448, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TFAM.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TFAM
  • Catalog：
    6448
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name TFAM Mouse mAb
Aliases TFAM; Transcription Factor A, Mitochondrial; TCF6L2; TCF6; Mitochondrial Transcription Factor 1; Transcription Factor 6; Transcription Factor 6-Like 2 (Mitochondrial Transcription Factor); Mitochondrial Transcription Factor A; Transcription Factor 6-Like 1; Transcription Factor 6-Like 3; Transcription Factor 6-Like 2; MTDPS15; TCF6L1; TCF6L3; MTTF1; MTTFA; MtTFA; MtTF1; TCF-6
Background

Gene Name: TFAM

NCBI Gene Entry: 7019

UniProt Entry: Q00059

Application Information

Molecular Weight: Predicted, 29 kDa; observed, 22 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9145

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human TFAM
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using TFAM antibody (Cat#6448). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TFAM antibody (Cat#6448, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with TFAM antibody (Cat#6448, 1:1,000). Nuclei were stained blue with DAPI; TFAM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of TFAM expression in HAP-1 cells using TFAM antibody (Cat#6448, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TFAM.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TFAM
  • Catalog：
    6448
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using TFAM antibody (Cat#6448). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TFAM antibody (Cat#6448, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with TFAM antibody (Cat#6448, 1:1,000). Nuclei were stained blue with DAPI; TFAM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TFAM expression in HAP-1 cells using TFAM antibody (Cat#6448, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TFAM.
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