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Home>Product>Primary Antibody

Phospho-PP2A Alpha (Y307) Recombinant Rabbit mAb

#1811

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Recombinant Antibody
Western blotting analysis using Phospho-PP2A alpha (Y307) antibody (Cat#1811). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-PP2A alpha (Y307) antibody (Cat#1811, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Phospho-PP2A alpha (Y307) antibody (Cat#1811, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PP2A alpha (Y307)  was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-PP2A alpha (Y307) expression in HAP-1 cells using Phospho-PP2A alpha (Y307) antibody (Cat#1811, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-PP2A alpha (Y307).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PPP2CA
  • Catalog：
    1811
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-PP2A Alpha (Y307) Recombinant Rabbit mAb
Aliases PPP2CA; Protein Phosphatase 2 Catalytic Subunit Alpha; Serine/Threonine-Protein Phosphatase 2A Catalytic Subunit Alpha Isoform; Protein Phosphatase 2, Catalytic Subunit, Alpha Isozyme; Replication Protein C; EC 3.1.3.16; PP2A-Alpha; PP2Calpha; PP2AC; RP-C; Protein Phosphatase 2 (Formerly 2A), Catalytic Subunit, Alpha Isoform; Protein Phosphatase 2A Catalytic Subunit, Alpha Isoform; PP2CALPHA; NEDLBA; PP2Ac; PP2CA; HJS3
Background

Gene Name: PPP2CA

NCBI Gene Entry: 5515

UniProt Entry: P67775

Application Information

Molecular Weight: Predicted, 36 kDa; observed, 35 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4580

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-PP2A alpha (Y307)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Phospho-PP2A alpha (Y307) antibody (Cat#1811). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-PP2A alpha (Y307) antibody (Cat#1811, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with Phospho-PP2A alpha (Y307) antibody (Cat#1811, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PP2A alpha (Y307)  was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Phospho-PP2A alpha (Y307) expression in HAP-1 cells using Phospho-PP2A alpha (Y307) antibody (Cat#1811, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-PP2A alpha (Y307).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PPP2CA
  • Catalog：
    1811
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Phospho-PP2A alpha (Y307) antibody (Cat#1811). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-PP2A alpha (Y307) antibody (Cat#1811, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Phospho-PP2A alpha (Y307) antibody (Cat#1811, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PP2A alpha (Y307)  was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-PP2A alpha (Y307) expression in HAP-1 cells using Phospho-PP2A alpha (Y307) antibody (Cat#1811, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-PP2A alpha (Y307).
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