Western blotting analysis using high mobility group box 2 antibody (Cat#4351). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with high mobility group box 2 antibody (Cat#4351, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of High mobility group box 2 expression in HepG2 cells using High mobility group box 2 antibody (Cat#4351, 1:2,000). Green, isotype control; red, High mobility group box 2.
Immunocytochemical staining of HepG2 cells with High mobility group box 2 antibody (Cat#4351, 1:1,000). Nuclei were stained blue with DAPI; High mobility group box 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
HMGB2; High Mobility Group Box 2; HMG2; High-Mobility Group (Nonhistone Chromosomal) Protein 2; High Mobility Group Protein B2; High Mobility Group Protein 2; HMG-2; High-Mobility Group Box 2
Western blotting analysis using high mobility group box 2 antibody (Cat#4351). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with high mobility group box 2 antibody (Cat#4351, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of High mobility group box 2 expression in HepG2 cells using High mobility group box 2 antibody (Cat#4351, 1:2,000). Green, isotype control; red, High mobility group box 2.
Immunocytochemical staining of HepG2 cells with High mobility group box 2 antibody (Cat#4351, 1:1,000). Nuclei were stained blue with DAPI; High mobility group box 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using high mobility group box 2 antibody (Cat#4351). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with high mobility group box 2 antibody (Cat#4351, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of High mobility group box 2 expression in HepG2 cells using High mobility group box 2 antibody (Cat#4351, 1:2,000). Green, isotype control; red, High mobility group box 2.
Immunocytochemical staining of HepG2 cells with High mobility group box 2 antibody (Cat#4351, 1:1,000). Nuclei were stained blue with DAPI; High mobility group box 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000762s ]
SELECT * FROM `goods` WHERE `code` = '4351' LIMIT 1 [ RunTime:0.001243s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000600s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000330s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001136s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5676 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000424s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001445s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5676 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000628s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000697s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000667s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.128424s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (10559,5948,4212,7778,3942,6347,6671,8015,37,5229) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002762s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000575s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000235s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.006365s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.002456s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001409s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000421s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000235s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.003440s ]
