Western blotting analysis using ING1 antibody (Cat#5647). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ING1 antibody (Cat#5647, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of ING1 expression in HT-1080 cells using ING1 antibody (Cat#5647, 1:2,000). Green, isotype control; red, ING1.
ING1; Inhibitor Of Growth Family Member 1; P33ING1b; P24ING1c; P47ING1a; P33ING1; P33; P47; Growth Inhibitory Protein ING1; Inhibitor Of Growth Protein 1; Tumor Suppressor ING1; Growth Inhibitor ING1; Inhibitor Of Growth 1
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human ING1
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using ING1 antibody (Cat#5647). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ING1 antibody (Cat#5647, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of ING1 expression in HT-1080 cells using ING1 antibody (Cat#5647, 1:2,000). Green, isotype control; red, ING1.
Western blotting analysis using ING1 antibody (Cat#5647). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ING1 antibody (Cat#5647, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of ING1 expression in HT-1080 cells using ING1 antibody (Cat#5647, 1:2,000). Green, isotype control; red, ING1.
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