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KD-Validated HLA-G Recombinant Rabbit mAb

#62191

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using HLA-G antibody (Cat#62191). HLA-G expression in wild type (WT) and HLA-G shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HLA-G antibody (Cat#62191, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    HLA-G
  • Catalog:
    62191
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated HLA-G Recombinant Rabbit mAb
Aliases HLA-G; Major Histocompatibility Complex, Class I, G; HLA Class I Histocompatibility Antigen, Alpha Chain G; HLA-G Histocompatibility Antigen, Class I, G; MHC Class I Antigen G; B2 Microglobulin; HLA G Antigen; Mutant MHC Class Ib Antigen; Mutant MHC Class I Antigen; MHC Class Ib Antigen; MHC Class I Protein; HLA-6.0; MHC-G; HLAG
Background

Gene Name: HLA-G

NCBI Gene Entry: 3135

UniProt Entry: P17693

Application Information

Molecular Weight: Predicted, 38 kDa; observed, 42 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB1760

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human HLA G
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using HLA-G antibody (Cat#62191). HLA-G expression in wild type (WT) and HLA-G shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HLA-G antibody (Cat#62191, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using HLA-G antibody (Cat#62191). HLA-G expression in wild type (WT) and HLA-G shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HLA-G antibody (Cat#62191, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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