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KRR1 Mouse mAb

#6270

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Western blotting analysis using KRR1 antibody (Cat#6270). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KRR1 antibody (Cat#6270, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with KRR1 antibody (Cat#6270,1:1,000). Nuclei were stained blue with DAPI; KRR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    KRR1
  • Catalog:
    6270
  • Application:
    WB, ICC
  • Species Reactivity:
    H
Information
Product Name KRR1 Mouse mAb
Aliases KRR1; KRR1 Small Subunit Processome Component Homolog; RIP-1; HRB2; KRR-R Motif-Containing Protein 1; HIV-1 Rev Binding Protein 2; HIV-1 Rev-Binding Protein 2; Rev-Interacting Protein 1; KRR1, Small Subunit (SSU) Processome Component, Homolog (Yeast); KRR1, Small Subunit (SSU) Processome Component, Homolog; KRR1, Small Subunit Processome Component Homolog; Rev Interacting Protein; Rip-1
Background

Gene Name: KRR1

NCBI Gene Entry: 11103

UniProt Entry: Q13601

Application Information

Molecular Weight: Predicted, 44 kDa; observed, 48 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB8280

Species Reactivity: Human

Applications Tested: Western Blotting (WB), immunocytochemistry (ICC)

Immunogen Recombinant protein of human KRR1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using KRR1 antibody (Cat#6270). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KRR1 antibody (Cat#6270, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with KRR1 antibody (Cat#6270,1:1,000). Nuclei were stained blue with DAPI; KRR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using KRR1 antibody (Cat#6270). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KRR1 antibody (Cat#6270, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with KRR1 antibody (Cat#6270,1:1,000). Nuclei were stained blue with DAPI; KRR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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