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Home>Product>Primary Antibody

KD-Validated Integrin Alpha V Recombinant Rabbit mAb

#63189

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using integrin subunit alpha V antibody (Cat#63189). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with integrin subunit alpha V antibody (Cat#63189, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using integrin subunit alpha V antibody (Cat#63189). Integrin subunit alpha V expression in wild-type (WT) and integrin subunit alpha V (ITGAV) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with integrin subunit alpha V antibody (Cat#63189, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of integrin subunit alpha V expression in HepG2 cells using integrin subunit alpha V antibody (Cat#63189, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, integrin subunit alpha V.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ITGAV
  • Catalog：
    63189
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated Integrin Alpha V Recombinant Rabbit mAb
Aliases ITGAV; Integrin Subunit Alpha V; CD51; MSK8; VNRA; VTNR; Integrin, Alpha V (Vitronectin Receptor, Alpha Polypeptide, Antigen CD51); Antigen Identified By Monoclonal Antibody L230; Vitronectin Receptor Subunit Alpha; Vitronectin Receptor; Integrin Alpha-V; Integrin AlphaVbeta3; Integrin, Alpha V; CD51 Antigen
Background

Gene Name: ITGAV

NCBI Gene Entry: 3685

UniProt Entry: P06756

Application Information

Molecular Weight: Predicted, 116 kDa; observed, 135 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4435

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human Integrin alpha V
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using integrin subunit alpha V antibody (Cat#63189). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with integrin subunit alpha V antibody (Cat#63189, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using integrin subunit alpha V antibody (Cat#63189). Integrin subunit alpha V expression in wild-type (WT) and integrin subunit alpha V (ITGAV) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with integrin subunit alpha V antibody (Cat#63189, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of integrin subunit alpha V expression in HepG2 cells using integrin subunit alpha V antibody (Cat#63189, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, integrin subunit alpha V.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ITGAV
  • Catalog：
    63189
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using integrin subunit alpha V antibody (Cat#63189). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with integrin subunit alpha V antibody (Cat#63189, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using integrin subunit alpha V antibody (Cat#63189). Integrin subunit alpha V expression in wild-type (WT) and integrin subunit alpha V (ITGAV) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with integrin subunit alpha V antibody (Cat#63189, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of integrin subunit alpha V expression in HepG2 cells using integrin subunit alpha V antibody (Cat#63189, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, integrin subunit alpha V.
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