Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

KD-Validated PRMT1 Recombinant Rabbit mAb

#63320

Click to copy product information

Return to List

Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using protein arginine methyltransferase 1 antibody (Cat#63320). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with protein arginine methyltransferase 1 antibody (Cat#63320, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using protein arginine methyltransferase 1 antibody (Cat#63320). Protein arginine methyltransferase 1 expression in wild-type (WT) and protein arginine methyltransferase 1 (PRMT1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with protein arginine methyltransferase 1 antibody (Cat#63320, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using protein arginine methyltransferase 1 antibody (Cat#63320, 1:1,000), Top panel: wild-type (WT); Bottom panal: protein arginine methyltransferase 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Protein arginine methyltransferase 1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of Protein arginine methyltransferase 1 expression in HeLa cells using Protein arginine methyltransferase 1 antibody (Cat#63320, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Protein arginine methyltransferase 1.
Validation of Protein arginine methyltransferase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Protein arginine methyltransferase 1 antibody (Cat#63320, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PRMT1
  • Catalog：
    63320
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated PRMT1 Recombinant Rabbit mAb
Aliases PRMT1; Protein Arginine Methyltransferase 1; HRMT1L2; HCP1; ANM1; HMT1 (HnRNP Methyltransferase, S. Cerevisiae)-Like 2; Histone-Arginine N-MethyltransferasePRMT1; Protein Arginine N-Methyltransferase 1; Interferon Receptor 1-Bound Protein 4; Highly Conserved Protein 1; IR1B4; Heterogeneous Nuclear Ribonucleoprotein Methyltransferase 1-Like 2; HMT1 HnRNP Methyltransferase-Like 2 (S. Cerevisiae); EC 2.1.1.319; EC 2.1.1.77; EC 2.1.1; HMT2
Background

Gene Name: PRMT1

NCBI Gene Entry: 3276

UniProt Entry: Q99873

Application Information

Molecular Weight: Predicted, 42 kDa; observed, 42 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5045

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human PRMT1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using protein arginine methyltransferase 1 antibody (Cat#63320). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with protein arginine methyltransferase 1 antibody (Cat#63320, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using protein arginine methyltransferase 1 antibody (Cat#63320). Protein arginine methyltransferase 1 expression in wild-type (WT) and protein arginine methyltransferase 1 (PRMT1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with protein arginine methyltransferase 1 antibody (Cat#63320, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HeLa cells using protein arginine methyltransferase 1 antibody (Cat#63320, 1:1,000), Top panel: wild-type (WT); Bottom panal: protein arginine methyltransferase 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Protein arginine methyltransferase 1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
  • Flow cytometric analysis of Protein arginine methyltransferase 1 expression in HeLa cells using Protein arginine methyltransferase 1 antibody (Cat#63320, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Protein arginine methyltransferase 1.
  • Validation of Protein arginine methyltransferase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Protein arginine methyltransferase 1 antibody (Cat#63320, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PRMT1
  • Catalog：
    63320
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using protein arginine methyltransferase 1 antibody (Cat#63320). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with protein arginine methyltransferase 1 antibody (Cat#63320, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using protein arginine methyltransferase 1 antibody (Cat#63320). Protein arginine methyltransferase 1 expression in wild-type (WT) and protein arginine methyltransferase 1 (PRMT1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with protein arginine methyltransferase 1 antibody (Cat#63320, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using protein arginine methyltransferase 1 antibody (Cat#63320, 1:1,000), Top panel: wild-type (WT); Bottom panal: protein arginine methyltransferase 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Protein arginine methyltransferase 1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of Protein arginine methyltransferase 1 expression in HeLa cells using Protein arginine methyltransferase 1 antibody (Cat#63320, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Protein arginine methyltransferase 1.
Validation of Protein arginine methyltransferase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Protein arginine methyltransferase 1 antibody (Cat#63320, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.