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Home>Product>Primary Antibody

KD-Validated ERBB2 Mouse mAb

#63444

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shRNA Knockdown Validated
Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444). Erb-b2 receptor tyrosine kinase 2 expression in wild type (WT) and erb-b2 receptor tyrosine kinase 2 (ERBB2) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Erb-b2 receptor tyrosine kinase 2 expression in H9c2 cells using Erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Erb-b2 receptor tyrosine kinase 2.
Validation of Erb-b2 receptor tyrosine kinase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) 293T cells were stained with Erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ERBB2
  • Catalog：
    63444
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated ERBB2 Mouse mAb
Aliases ERBB2; Erb-B2 Receptor Tyrosine Kinase 2; HER2; NEU 2; C-ERB-2; C-ERB2; MLN-19; HER-2; CD340; NGL; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2; Tyrosine Kinase-Type Cell Surface Receptor HER2; Neuro/Glioblastoma Derived Oncogene Homolog; Human Epidermal Growth Factor Receptor 2; Receptor Tyrosine-Protein Kinase ErbB-2; Metastatic Lymph Node Gene 19 Protein; Proto-Oncogene C-ErbB-2; Proto-Oncogene Neu; P185(ErbB2); EC 2.7.10.1; MLN 19; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2 (Neuro/Glioblastoma Derived Oncogene Homolog) 2; V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncoprotein 2; Neuroblastoma/Glioblastoma Derived Oncogene Homolog; Metastatic Lymph Node Gene 19; C-Erb B2/Neu Protein; CD340 Antigen; P185(ERBB2); HER-2/Neu; Herstatin; P185erbB2; EC 2.7.10; VSCN2; MLN19; TKR1
Background

Gene Name: ERBB2

NCBI Gene Entry: 2064

UniProt Entry: P04626

Application Information

Molecular Weight: Predicted, 138 kDa; observed, 180 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB5655

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human ERBB2
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB):1:2,500-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
  • Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444). Erb-b2 receptor tyrosine kinase 2 expression in wild type (WT) and erb-b2 receptor tyrosine kinase 2 (ERBB2) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Erb-b2 receptor tyrosine kinase 2 expression in H9c2 cells using Erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Erb-b2 receptor tyrosine kinase 2.
  • Validation of Erb-b2 receptor tyrosine kinase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) 293T cells were stained with Erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ERBB2
  • Catalog：
    63444
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444). Erb-b2 receptor tyrosine kinase 2 expression in wild type (WT) and erb-b2 receptor tyrosine kinase 2 (ERBB2) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Erb-b2 receptor tyrosine kinase 2 expression in H9c2 cells using Erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Erb-b2 receptor tyrosine kinase 2.
Validation of Erb-b2 receptor tyrosine kinase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) 293T cells were stained with Erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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