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Home>Product>Primary Antibody

KD-Validated NEFM Mouse mAb

#63480

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shRNA Knockdown Validated
Western blotting analysis using neurofilament medium chain antibody (Cat#63480). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with neurofilament medium chain antibody (Cat#63480, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using neurofilament medium chain antibody (Cat#63480). Neurofilament medium chain expression in wild type (WT) and neurofilament medium chain (NEFM) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with neurofilament medium chain antibody (Cat#63480, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human skin tissue using neurofilament medium chain antibody (Cat#63480, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HeLa cells with Neurofilament medium chain antibody (Cat#63480, 1:1,000). Nuclei were stained blue with DAPI; Neurofilament medium chain was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using Neurofilament medium chain antibody (Cat#63480, 1:1,000), Top panel: wild-type (WT); Bottom panal: Neurofilament medium chain shRNA knockdown (KD). Nuclei were stained blue with DAPI; Neurofilament medium chain was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of neurofilament medium chain expression in HeLa cells using neurofilament medium chain antibody (Cat#63480, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, neurofilament medium chain.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NEFM
  • Catalog：
    63480
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated NEFM Mouse mAb
Aliases NEFM; Neurofilament Medium Chain; NF-M; NFM; NEF3; Neurofilament, Medium Polypeptide 150kDa; Neurofilament Medium Polypeptide; Neurofilament Triplet M Protein; 160 KDa Neurofilament Protein; Neurofilament 3; Neurofilament, Medium Polypeptide; Neurofilament-3 (150 KD Medium); Neurofilament Medium
Background

Gene Name: NEFM

NCBI Gene Entry: 4741

UniProt Entry: P07197

Application Information

Molecular Weight: Predicted, 102 kDa; observed, 150 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB5825

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen Recombinant protein of human NEFM
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB):1:2,500-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using neurofilament medium chain antibody (Cat#63480). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with neurofilament medium chain antibody (Cat#63480, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using neurofilament medium chain antibody (Cat#63480). Neurofilament medium chain expression in wild type (WT) and neurofilament medium chain (NEFM) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with neurofilament medium chain antibody (Cat#63480, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human skin tissue using neurofilament medium chain antibody (Cat#63480, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HeLa cells with Neurofilament medium chain antibody (Cat#63480, 1:1,000). Nuclei were stained blue with DAPI; Neurofilament medium chain was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Immunocytochemical staining of HeLa cells using Neurofilament medium chain antibody (Cat#63480, 1:1,000), Top panel: wild-type (WT); Bottom panal: Neurofilament medium chain shRNA knockdown (KD). Nuclei were stained blue with DAPI; Neurofilament medium chain was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of neurofilament medium chain expression in HeLa cells using neurofilament medium chain antibody (Cat#63480, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, neurofilament medium chain.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NEFM
  • Catalog：
    63480
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using neurofilament medium chain antibody (Cat#63480). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with neurofilament medium chain antibody (Cat#63480, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using neurofilament medium chain antibody (Cat#63480). Neurofilament medium chain expression in wild type (WT) and neurofilament medium chain (NEFM) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with neurofilament medium chain antibody (Cat#63480, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human skin tissue using neurofilament medium chain antibody (Cat#63480, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HeLa cells with Neurofilament medium chain antibody (Cat#63480, 1:1,000). Nuclei were stained blue with DAPI; Neurofilament medium chain was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using Neurofilament medium chain antibody (Cat#63480, 1:1,000), Top panel: wild-type (WT); Bottom panal: Neurofilament medium chain shRNA knockdown (KD). Nuclei were stained blue with DAPI; Neurofilament medium chain was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of neurofilament medium chain expression in HeLa cells using neurofilament medium chain antibody (Cat#63480, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, neurofilament medium chain.
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